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Multi parametric imaging

This platform of d'Alembert Institute allows the study of the fluorescence of solid samples under a microscope. These samples can be amorphous or crystalline, neat or heterogeneous. They have to fit the observation condition of an inverted fluorescence microscope for biology. Solid state fluorescence is our main research domain but the reactivity in microfluidic flows and the cellular imaging are part of our expertise.

We can measure :

  • Stationary absorption and fluorescence spectra. (F>20µm, 400nm-800nm)
  • Dichroism and birefringence by transmission.
  • Fluorescence lifetime imaging FLIM and record movies of it. (F>1µm, 400nm-800nm)
  • Time resolved fluorescence spectra along a line of the sample SLIM.
  • Time resolved fluorescence anisotropy imaging  AFLIM.

We can further study the relaxation of the system after a perturbation : by light, electricity or heat.
For that, the imaging platform is equipped with two inverted microscopes for epifluorescence, a full time correlated single photon counting line coupled with two lasers and a QA-MCP-Photomultiplyer. This photomultiplier is able to count photons one by one with a time resolution of 60ps and a spatial resolution of 1024x1024. The photon counting electronic can record for each photon, its origin in the sample, its fluorescence delay (60ps-100ns), its arrival time (100ns-hours) and the state of the stimulus. The data are saved for each photon. The analysis proceeds by successive runs, focusing progressively on the actual response provided by the sample. It is not rare to observe and analyse something else than the original project.
We have developed our expertise in the analysis of FLIM images. We can build maps of concentrations in minutes from the hundred thousand decays that we collect from each image.
We have developed an expertise in the analysis of the decays in the solid phase. The complexity of these decays comes in a large part from the few quenchers that are surrounding a fluorescent molecule. We know now how to handle this complexity.

Recent Researches :

  • LIGHTER is a project sponsored by IDA in 2015/16 leaded by Claude Nogues on the study of gold nanoparticles able to thermally release DNA strands.
  • NESSYNED is an ANR grant on the detection of ionizing radiations for which the study TADF molecules.
  • NANO THERMO LIP: Timothée Labouret has observed and studied the formation of a light plasma during the interaction of a pulsed laser with gold nanoparticles. Beyond the interpretation of a common secondary effect of confocal microscope, the plasma comes with the formation of ROS molecules that open the way to an application in the domain of the light therapy of cancers. (Labouret, T. et al., Small 2015, 11, 4475-4479).


Jean-Frédéric Audibert
Tel : - 53.56

Equipments list:

 - 2 inverted microscopes
TE2000, Nikon
- T Pulse-200, Yb:KYW ,
1030 nm @ 400 fs @ 10 MHz, SHG@515nm, THG@343nm, Amplitude System
- Pulse picker,
gamme 5MHz - 1kHz, Amplitude System
- Continuum Laser SMHP-40.2-A-PP-RC,
400nm-800nm @ 10MHz, LEUKOS
- Spectrometer MAYA2000PRO,
200 nm - 1100 nm, resolution 6 nm, Ocean Optics
- CCD CoolSnap HQ2 Monochrome,
6.45µm x 6.45µm, 1394x1040 pxl, ADC @10-20MHz, High QE [450nm - 600nm] > 60 % , Ropert Scientific
Time and Space Correlated Single Photon Counting, active area f=25 mm, resolution 40 µm on ship, codage XxY=1024x1024, temporal  resolution 50 ps, count rate maximum 300 kHz, resolution macro-time 10ns, TAC window 7ns - 100ns, 12 bits,
Leibniz Institute for Neurobiology Magdeburg ,Germany, Yury Prokazov, Evgeny Turbin and Werner Zuschratter 


    Bâtiment de l'Institut d'Alembert - Rez-de-chaussée bas (B37)