We can further study the relaxation of the system after a perturbation : by light, electricity or heat.
For that, the imaging platform is equipped with two inverted microscopes for epifluorescence, a full time correlated single photon counting line coupled with two lasers and a QA-MCP-Photomultiplyer. This photomultiplier is able to count photons one by one with a time resolution of 60ps and a spatial resolution of 1024x1024. The photon counting electronic can record for each photon, its origin in the sample, its fluorescence delay (60ps-100ns), its arrival time (100ns-hours) and the state of the stimulus. The data are saved for each photon. The analysis proceeds by successive runs, focusing progressively on the actual response provided by the sample. It is not rare to observe and analyse something else than the original project.
We have developed our expertise in the analysis of FLIM images. We can build maps of concentrations in minutes from the hundred thousand decays that we collect from each image.
We have developed an expertise in the analysis of the decays in the solid phase. The complexity of these decays comes in a large part from the few quenchers that are surrounding a fluorescent molecule. We know now how to handle this complexity.