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Rapid and accurate detection of Escherichia coli growth by fluorescent pH-sensitive organic nanoparticles for High-throughput screening applications.

Si, Y., Grazon, C., Clavier, G., Rieger, J., Audibert, J. F., Sclavi, B., & Meallet-Renault, R. (2015). Biosensors and Bioelectronics, 75.

Rapid detection of bacterial growth is an important issue in the food industry and for medical research. Here we present a novel kind of pH-sensitive fluorescent nanoparticles (FANPs) that can be used for the rapid and accurate real-time detection of Escherichia coli growth. These organic particles are designed to be non-toxic and highly water-soluble. The coupling of pH sensitive fluoresceinamine to the nanoparticles results in an increased sensitivity to changes in pH within a physiologically relevant range that can be used to monitor the presence of live bacteria by continuous monitoring of bacterial growth via real-time detection over long time scales in small volumes and can thus be used for the screening of a large number of samples for high-throughput applications such as screening for the presence of antibiotic resistant strains.