The aim of this projet is to liberate genetic material from the inside of a biological cell thanks to two optical controls. For this, it relies upon the photonic and photo thermal properties associated with the localised plasmon phenomena in the nanoparticles of gold. A single DNA strand is grafted onto a nanoparticle. another strand is attached to it via a conjugated sequence. The localised warning brought about by the lighting up of the nanoparticule causes this strand to become detached. Other researchers have already shown the feasibility of this method, but without controlling the quantity of genetic material liberated and without an in vivo demonstration. We propose optimising the conditions os the light irradiation in order to quantify in real time the intracellular loss both in vitro and in vivo of the single strand DNA.

There is a lot of at stake for genetic therapy with regards to the controlled intracellular dispersal of genetic. A multidisciplinary approach such as the one we are putting in place aapears to be appropriate to deal with the problems concerning the making and characterisation of the "nano-tools", with their response to light and heat stimulation, to imagery and to the biological response.

For this sort of research, the daily practice of interdisciplinarity as carried out at the Alembert Institute - in our case bringing together physicians, chemists and biologists - is an undeniable advantage. The initial financing of the Alembert Institute allowed us to obtain thesis financing from the IDEX paris-Saclay. Our doctorate, issuing from the Master Monabiphot, strated work with us last september. The work we do for LIGhTER also benefits our other multodisciplinary projects with a biomedical aim.